| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 2 | Ava GIBSON | 14 | Prescot | 3:51.17 |
| 2 | 4 | Lauren WATKINS | 14 | Prescot | 3:25.99 |
| 3 | 6 | Madison MINARDS | 13 | Prescot | 3:22.18 |
| 4 | 5 | Chloe JONES | 11 | Prescot | 3:25.90 |
| 5 | 3 | Isabella DYER | 11 | Prescot | 3:45.80 |
| 6 | 1 | Mollie MCNALLY | 9 | Prescot | |